thr 77 No Further a Mystery
thr 77 No Further a Mystery
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Antibodies bound to the blocking peptide not bind to the epitope to the target protein. By evaluating the staining within the blocked antibody vs . the antibody alone, one can see which staining is distinct.
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This information outlines further more probable optimization of cellular and immunoassay parameters to guarantee the very best results are...
This tutorial outlines additional feasible optimization of cellular and immunoassay parameters to be certain the absolute best final results are...
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The antibody is from purified rabbit serum by affinity purification by way of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by both of those antibodies...
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Blocking peptides are peptides that bind specifically on the target antibody and block antibody binding. These peptide commonly includes the epitope regarded because of the antibody.
Goal Information and facts Cyclin E belongs towards the hugely conserved cyclin family, whose members are characterized by a extraordinary periodicity in protein abundance with the cell cycle. Cyclins purpose as regulators of CDK kinases. Diverse cyclins show distinctive expression and degradation styles which add for the temporal coordination of each mitotic occasion. Cyclin E kinds a fancy with and features to be a regulatory subunit of CDK2, whose exercise is needed for mobile cycle G1/S changeover. This protein accumulates with the G1-S stage boundary and it is degraded as cells progress as a result of S phase.
Overexpression of the gene continues to be observed in many tumors, which ends up in chromosome instability, and therefore may well lead to tumorigenesis. This protein was uncovered to associate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped into the ATM locus), which participates in cell-cycle controlled histone gene expression and performs a vital purpose in promoting mobile-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinct isoforms, are actually explained. Two more splice variants have been documented but in-depth nucleotide sequence info isn't nevertheless out there.
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